Humasis COVID-19 IgG/IgM Test is a rapid immunochromatographic assay test which detects IgG and IgM antibody to COVID-19 in human blood.
A nitrocellulose membrane strip in the device contains two test lines (G and M line) and a control line (C). G line is pre-coated with mouse anti-human IgG for detection of IgG anti-COVID-19, and M line is pre-coated with mouse anti-human IgM for detection of IgM anti-COVID-19. C (Control line) is coated with goat anti-mouse IgG.
When sample is added to sample pad, it moves through the conjugate pad, where recombinant antigen-colloidal gold particle will react with the IgG and IgM antibodies specific to COVID-19 in the sample, forming an immunocomplex. The complex moves along the membrane by capillary action and makes contact with the immobilised antibody coated in the test region.
Coloured line in the test region indicates a positive result for coronavirus. The absence of coloured line in the test region suggests a negative result. The complex continues to move to the control region and will react with immobilised reagents that capture coloured conjugate regardless of test specimen composition. The resulting visible coloured line in the control region confirms that the assay is functioning correctly and its result is valid.